Pdms Microchip Electrophoresis with High Separation Efficiency by Simple and Quick Modification of Phospholipid Polymer
نویسندگان
چکیده
This paper reports the high separation efficiency zone-electrophoresis of proteins in poly(dimethylsiloxane) (PDMS) microchip coated with the physically adsorbed phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-3-(methacryloyloxy)propyltris(trimethylsiloxy)silane (MPTSSi)) (PMMSi), using the simple and quick dip coating method. High suppression of protein adsorption and reduction of electroosmotic flow (EOF) were achieved by increasing hydrophilicity, and decreasing the surface -potential of PDMS microchannels. Furthermore, proteins were completely separated within 30 seconds. It was confirmed that the durable and reproducible membrane of PMMSi could be formed on the PDMS surface, and it was applied for microchip electrophoresis (MCE) with high separation efficiency.
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